Paul O'Connor and Nicole Foley, iPiPE Interns in the 2017 Apple (New York/New England) Crop-Pest Program have created an interesting video showing psudothecium removal from an apple leaf (
Using a small hook, the leaf's cuticle is peeled back, allowing access to the pseudothecium itself, which is then placed on a slide, topped with a coverslip and gently squashed to reveal the asci housed within it. Ten pseudothecia per slide (two pseudothecia from each leaf of a five leaf sample) are removed, and analyzed for ascus maturity and spore count under greater magnification.